Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Centrosomal protein TRIM43 restricts herpesvirus infection by regulating nuclear lamina integrity.

Full F, van Gent M, Sparrer KMJ, Chiang C, Zurenski MA, Scherer M, Brockmeyer NH, Heinzerling L, Stuerzl M, Korn K, Stamminger T, Ensser A, Gack MU

Tripartite motif (TRIM) proteins mediate antiviral host defences by either directly targeting viral components or modulating innate immune responses. Here we identify a mechanism of antiviral restriction in which a TRIM E3 ligase controls viral replication by regulating the structure of host cell centrosomes and thereby nuclear lamina integrity. Through RNAi screening we identified several TRIM proteins, including TRIM43, that ... [more]

Nat Microbiol Dec. 01, 2018; 4(1);164-176 [Pubmed: 30420784]

Throughput

  • High Throughput

Curated By

  • BioGRID