BAIT

IKZF2

ANF1A2, HELIOS, ZNF1A2, ZNFN1A2
IKAROS family zinc finger 2 (Helios)
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens
PREY

HDAC2

HD2, RPD3, YAF1
histone deacetylase 2
GO Process (29)
GO Function (13)
GO Component (8)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A molecular dissection of the repression circuitry of Ikaros.

Koipally J, Georgopoulos K

Ikaros is a key regulator of the hemo-lymphoid system in which it is presumed to function by both potentiating and repressing gene expression. Repression is mediated through two independent domains at the N and C terminus of the protein, both of which can independently recruit the corepressors Mi-2beta, Sin3A, and Sin3B and the Class I histone deacetylases 1 and 2; ... [more]

J. Biol. Chem. Aug. 02, 2002; 277(31);27697-705 [Pubmed: 12015313]

Throughput

  • Low Throughput

Curated By

  • BioGRID