An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

A structural model for elongation factor 1 (EF-1) and phosphorylation by protein kinase CKII.

Sheu GT, Traugh JA

EF-1alpha binds aminoacyl-tRNA to the ribosome with the hydrolysis of GTP; the betagammadelta complex facilitates the exchange of GDP for GTP to initiate another round of elongation. To examine the subunit structure of EF-1 and phosphorylation by protein kinase CKII, recombinant beta, gamma, and delta subunits from rabbit were expressed in E. coli and the subunits were reconstituted into partial ... [more]

Mol. Cell. Biochem. Jan. 01, 1999; 191(1);181-6 [Pubmed: 10094407]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
EEF1B2 EEF1B2	Cross-Linking-MS (XL-MS) Cross-Linking-MS (XL-MS) An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).	Kaake RM (2014)	High	-	BioGRID	3729911

Curated By

BioGRID

Interaction Summary

EEF1B2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]translation elongation factor activity [NAS]

Gene Ontology Cellular Component