An interaction is detected between two proteins using chemically reactive or photo-activatable cross-linking reagents that covalently link amino acids in close proximity, followed by mass spectrometry analysis to identify the linked peptides (reviewed in PMID 37406423, 37104977). Experiments may be carried with live cells or cell lysates in which all proteins are expressed at endogenous levels (e.g. PMID 34349018, 35235311) or with recombinant proteins (e.g., PMID 28537071).

Publication

A new in vivo cross-linking mass spectrometry platform to define protein-protein interactions in living cells.

Kaake RM, Wang X, Burke A, Yu C, Kandur W, Yang Y, Novtisky EJ, Second T, Duan J, Kao A, Guan S, Vellucci D, Rychnovsky SD, Huang L

Protein-protein interactions (PPIs) are fundamental to the structure and function of protein complexes. Resolving the physical contacts between proteins as they occur in cells is critical to uncovering the molecular details underlying various cellular activities. To advance the study of PPIs in living cells, we have developed a new in vivo cross-linking mass spectrometry platform that couples a novel membrane-permeable, ... [more]

Mol Cell Proteomics Dec. 01, 2014; 13(12);3533-43 [Pubmed: 25253489]

Throughput

High Throughput

Additional Notes

In Vivo Azide-A-DSBSO Cross-linking of HEK 293 Cells

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
EEF1B2 EEF1B2	Co-purification Co-purification An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.	Sheu GT (1999)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

EEF1B2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]translation elongation factor activity [NAS]

Gene Ontology Cellular Component