SQSTM1
Gene Ontology Biological Process
- apoptotic signaling pathway [TAS]
- autophagy [IMP, TAS]
- endosomal transport [TAS]
- intracellular signal transduction [TAS]
- macroautophagy [ISS]
- negative regulation of apoptotic process [TAS]
- neurotrophin TRK receptor signaling pathway [TAS]
- positive regulation of apoptotic process [TAS]
- positive regulation of macroautophagy [IMP]
- positive regulation of transcription from RNA polymerase II promoter [TAS]
- protein localization [TAS]
- protein phosphorylation [NAS]
- regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- regulation of Ras protein signal transduction [NAS]
- response to stress [TAS]
- ubiquitin-dependent protein catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
RIPK1
Gene Ontology Biological Process
- MyD88-independent toll-like receptor signaling pathway [TAS]
- T cell apoptotic process [ISS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- activation of JUN kinase activity [TAS]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [TAS]
- amyloid fibril formation [IMP]
- apoptotic process [IMP, TAS]
- apoptotic signaling pathway [TAS]
- cellular protein catabolic process [IDA]
- cellular response to tumor necrosis factor [IDA]
- extrinsic apoptotic signaling pathway [IDA, IMP]
- innate immune response [TAS]
- necroptotic process [IMP]
- necroptotic signaling pathway [IMP, ISS]
- negative regulation of I-kappaB kinase/NF-kappaB signaling [IMP]
- negative regulation of extrinsic apoptotic signaling pathway [IMP]
- peptidyl-serine autophosphorylation [IDA]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA, IEP]
- positive regulation of JNK cascade [IDA]
- positive regulation of NF-kappaB transcription factor activity [IMP]
- positive regulation of apoptotic process [IDA, IMP]
- positive regulation of extrinsic apoptotic signaling pathway [IMP]
- positive regulation of interleukin-8 production [IDA]
- positive regulation of macrophage differentiation [IMP]
- positive regulation of necroptotic process [IMP]
- positive regulation of programmed cell death [IMP]
- positive regulation of protein phosphorylation [IMP]
- positive regulation of reactive oxygen species metabolic process [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IDA]
- positive regulation of tumor necrosis factor production [IDA]
- positive regulation of type I interferon production [TAS]
- protein autophosphorylation [IDA]
- protein heterooligomerization [IMP]
- protein homooligomerization [IDA]
- regulation of ATP:ADP antiporter activity [IMP]
- regulation of extrinsic apoptotic signaling pathway in absence of ligand [TAS]
- response to tumor necrosis factor [IMP]
- ripoptosome assembly [IMP]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
- tumor necrosis factor-mediated signaling pathway [IC]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Sorafenib-induced defective autophagy promotes cell death by necroptosis.
Autophagy is one of the main cytoprotective mechanisms that cancer cells deploy to withstand the cytotoxic stress and survive the lethal damage induced by anti-cancer drugs. However, under specific conditions, autophagy may, directly or indirectly, induce cell death. In our study, treatment of the Atg5-deficient DU145 prostate cancer cells, with the multi-tyrosine kinase inhibitor, sorafenib, induces mitochondrial damage, autophagy and ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RIPK1 SQSTM1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | 1666 | BioGRID | 3483812 | |
| SQSTM1 RIPK1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| RIPK1 SQSTM1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| SQSTM1 RIPK1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 3736824 | |
| SQSTM1 RIPK1 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID