PREY

KCNJ6

BIR1, GIRK-2, GIRK2, KATP-2, KATP2, KCNJ7, KIR3.2, hiGIRK2
potassium inwardly-rectifying channel, subfamily J, member 6
Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Structure-Activity Relationship of NF023 Derivatives Binding to XIAP-BIR1.

Sorrentino L, Cossu F, Milani M, Malkoc B, Huang WC, Tsay SC, Ru Hwu J, Mastrangelo E

Inhibitors of Apoptosis Proteins (IAPs) are conserved E3-ligases that ubiquitylate substrates to prevent apoptosis and activate the NF-kB survival pathway, often deregulated in cancer. IAPs-mediated regulation of NF-kB signaling is based on the formation of protein complexes by their type-I BIR domains. The XIAP-BIR1 domain dimerizes to bind two TAB1 monomers, leading to downstream NF-kB activation. Thus, impairment of XIAP-BIR1 ... [more]

ChemistryOpen Apr. 01, 2019; 8(4);476-482 [Pubmed: 31011505]

Throughput

  • Low Throughput

Additional Notes

  • assayed using SEC (size exclusion chromatography)

Curated By

  • BioGRID