BAIT

LCK

IMD22, LSK, YT16, p56lck, pp58lck, RP4-675E8.4
LCK proto-oncogene, Src family tyrosine kinase
GO Process (30)
GO Function (14)
GO Component (7)
Homo sapiens
PREY

SKAP2

PRAP, RA70, SAPS, SCAP2, SKAP-HOM, SKAP55R
src kinase associated phosphoprotein 2
GO Process (3)
GO Function (1)
GO Component (4)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

Publication

The SRC-family tyrosine kinase HCK shapes the landscape of SKAP2 interactome.

Bureau JF, Cassonnet P, Grange L, Dessapt J, Jones L, Demeret C, Sakuntabhai A, Jacob Y

The SRC Kinase Adaptor Phosphoprotein 2 (SKAP2) is a broadly expressed adaptor associated with the control of actin-polymerization, cell migration, and oncogenesis. After activation of different receptors at the cell surface, this dimeric protein serves as a platform for assembling other adaptors such as FYB and some SRC family kinase members, although these mechanisms are still poorly understood. The goal ... [more]

Oncotarget Mar. 02, 2018; 9(17);13102-13115 [Pubmed: 29568343]

Throughput

  • Low Throughput

Curated By

  • BioGRID