USP1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- nucleoplasm [TAS]
- nucleus [IDA]
EZH2
Gene Ontology Biological Process
- chromatin organization [TAS]
- histone H3-K27 methylation [IDA]
- negative regulation of gene expression, epigenetic [IDA]
- negative regulation of retinoic acid receptor signaling pathway [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IMP]
- positive regulation of MAP kinase activity [IDA]
- positive regulation of Ras GTPase activity [IDA]
- positive regulation of epithelial to mesenchymal transition [IDA]
- positive regulation of protein serine/threonine kinase activity [IDA]
- regulation of circadian rhythm [IMP]
- regulation of transcription, DNA-templated [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Biochemical Activity (Deubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
Aberrant Activation of ?-Catenin Signaling Drives Glioma Tumorigenesis via USP1-Mediated Stabilization of EZH2.
Aberrant activation of ?-catenin signaling is a critical driver for tumorigenesis, but the mechanism underlying this activation is not completely understood. In this study, we demonstrate a critical role of ?-catenin signaling in stabilization of enhancer of zeste homolog 2 (EZH2) and control of EZH2-mediated gene repression in oncogenesis. ?-Catenin/TCF4 activated the transcription of the deubiquitinase USP1, which then interacted ... [more]
Throughput
- Low Throughput
Additional Notes
- in vitro deubiquitination with USP1 as deubiquitinase, WDR48 as a supplementary component and EZH2 as substrate
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| USP1 EZH2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| EZH2 USP1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID