BAIT
JNM1
INS1, PAC3, L000000884, YMR294W
Component of the yeast dynactin complex; consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B
GO Process (2)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
PREY
YPK1
SLI2, serine/threonine protein kinase YPK1, L000002541, YKL126W
Serine/threonine protein kinase; phosphorylates and downregulates flippase activator Fpk1p; inactivates Orm1p and Orm2p (inhibitors of serine:palmitoyl-coenzyme A transferase) by phosphorylation in response to compromised sphingolipid synthesis; mutations affect receptor-mediated endocytosis and sphingolipid-mediated and cell integrity signaling pathways; homolog of mammalian kinase SGK; YPK1 has a paralog, YPK2, that arose from the whole genome duplication
GO Process (7)
GO Function (1)
GO Component (3)
Gene Ontology Biological Process
- eisosome assembly [IGI]
- glycerophospholipid metabolic process [IGI, IMP]
- negative regulation of phospholipid translocation [IMP]
- negative regulation of sphingolipid biosynthesis involved in cellular sphingolipid homeostasis [IMP]
- protein autophosphorylation [IDA]
- protein phosphorylation [IDA, IMP]
- response to pheromone involved in conjugation with cellular fusion [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Saccharomyces cerevisiae (S288c)
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Annotating novel genes by integrating synthetic lethals and genomic information.
BACKGROUND: Large scale screening for synthetic lethality serves as a common tool in yeast genetics to systematically search for genes that play a role in specific biological processes. Often the amounts of data resulting from a single large scale screen far exceed the capacities of experimental characterization of every identified target. Thus, there is need for computational tools that select ... [more]
Unknown Jan. 16, 2008; 2(0);3 [Pubmed: 18194531]
Throughput
- High Throughput
Ontology Terms
- phenotype: inviable (APO:0000112)
Additional Notes
- High Throughput: Synthetic Genetic Array (SGA) analysis
Curated By
- BioGRID