BAIT

CUL1

ATCUL1, AUXIN RESISTANT 6, AXR6, T10P11.26, T10P11_26, cullin 1, AT4G02570
cullin 1
Arabidopsis thaliana (Columbia)
PREY

EBF2

EIN3-binding F box protein 2, F18G18.90, F18G18_90, AT5G25350
EIN3-binding F-box protein 2
GO Process (3)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Arabidopsis thaliana (Columbia)

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

A new CULLIN 1 mutant has altered responses to hormones and light in Arabidopsis.

Moon J, Zhao Y, Dai X, Zhang W, Gray WM, Huq E, Estelle M

Regulated protein degradation contributes to plant development by mediating signaling events in many hormone, light, and developmental pathways. Ubiquitin ligases recognize and ubiquitinate target proteins for subsequent degradation by the 26S proteasome. The multisubunit SCF is the best-studied class of ubiquitin ligases in Arabidopsis (Arabidopsis thaliana). However, the extent of SCF participation in signaling networks is unclear. SCFs are composed ... [more]

Plant Physiol. Feb. 01, 2007; 143(2);684-96 [Pubmed: 17158585]

Throughput

  • Low Throughput

Additional Notes

  • Enhances ethylene sensitivity.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
EBF2 CUL1
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
-

Curated By

  • BioGRID