BAIT

CACNA1A

APCA, BI, Caca1a, Cacnl1a4, Cav2.1, Ccha1a, EA2, FHM, HPCA, MHP, MHP1, SCA6, alpha1A, la, nmf352, rkr, tg
calcium channel, voltage-dependent, P/Q type, alpha 1A subunit
GO Process (50)
GO Function (5)
GO Component (11)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus
PREY

CACNB2

AW060387, CAB2, Cavbeta2, Cchb2, RP23-56A7.2
calcium channel, voltage-dependent, beta 2 subunit
Mus musculus

Co-fractionation

Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

BraInMap Elucidates the Macromolecular Connectivity Landscape of Mammalian Brain.

Pourhaghighi R, Ash PEA, Phanse S, Goebels F, Hu LZM, Chen S, Zhang Y, Wierbowski SD, Boudeau S, Moutaoufik MT, Malty RH, Malolepsza E, Tsafou K, Nathan A, Cromar G, Guo H, Abdullatif AA, Apicco DJ, Becker LA, Gitler AD, Pulst SM, Youssef A, Hekman R, Havugimana PC, White CA, Blum BC, Ratti A, Bryant CD, Parkinson J, Lage K, Babu M, Yu H, Bader GD, Wolozin B, Emili A

Connectivity webs mediate the unique biology of the mammalian brain. Yet, while cell circuit maps are increasingly available, knowledge of their underlying molecular networks remains limited. Here, we applied multi-dimensional biochemical fractionation with mass spectrometry and machine learning to survey endogenous macromolecules across the adult mouse brain. We defined a global "interactome" comprising over one thousand multi-protein complexes. These include ... [more]

Cell Syst Apr. 22, 2020; 10(4);333-350.e14 [Pubmed: 32325033]

Quantitative Score

  • 0.98 [EPIC Score]

Throughput

  • High Throughput

Additional Notes

  • High confidence interactions had an EPIC Score >= 0.683

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CACNA1A CACNB2
Affinity Capture-Western
Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Low-BioGRID
-
CACNB2 CACNA1A
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID