BAIT

IGF2BP1

CRD-BP, CRDBP, IMP-1, IMP1, VICKZ1, ZBP1
insulin-like growth factor 2 mRNA binding protein 1
Homo sapiens
PREY

FLOT1

DADB-118P11.2
flotillin 1
Homo sapiens

Affinity Capture-RNA

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

p62/SQSTM1 Fuels Melanoma Progression by Opposing mRNA Decay of a Selective Set of Pro-metastatic Factors.

Karras P, Riveiro-Falkenbach E, Canon E, Tejedo C, Calvo TG, Martinez-Herranz R, Alonso-Curbelo D, Cifdaloz M, Perez-Guijarro E, Gomez-Lopez G, Ximenez-Embun P, Munoz J, Megias D, Olmeda D, Moscat J, Ortiz-Romero PL, Rodriguez-Peralto JL, Soengas MS

Modulators of mRNA stability are not well understood in melanoma, an aggressive tumor with complex changes in the transcriptome. Here we report the ability of p62/SQSTM1 to extend mRNA half-life of a spectrum of pro-metastatic factors. These include FERMT2 and other transcripts with no previous links to melanoma. Transcriptomic, proteomic, and interactomic analyses, combined with validation in clinical biopsies and ... [more]

Cancer Cell Dec. 14, 2018; 35(1);46-63.e10 [Pubmed: 30581152]

Throughput

  • Low Throughput

Additional Notes

  • interaction of the protein corresponding to the bait gene IGF2BP1 with mRNA corresponding to the indicated hit genes, assayed using RIP (RNA immunoprecipitation) followed by RT-qPCR (real time quantitative polymerase chain reaction)

Curated By

  • BioGRID