An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.

Publication

A translational silencing function of MCPIP1/Regnase-1 specified by the target site context.

Behrens G, Winzen R, Rehage N, Doerrie A, Barsch M, Hoffmann A, Hackermueller J, Tiedje C, Heissmeyer V, Holtmann H

The expression of proteins during inflammatory and immune reactions is coordinated by post-transcriptional mechanisms. A particularly strong suppression of protein expression is exerted by a conserved translational silencing element (TSE) identified in the 3' UTR of NFKBIZ mRNA, which is among the targets of the RNA-binding proteins Roquin-1/2 and MCPIP1/Regnase-1. We present evidence that in the context of the TSE ... [more]

Nucleic Acids Res. Dec. 04, 2017; 46(8);4256-4270 [Pubmed: 29471506]

Throughput

Low Throughput

Additional Notes

assayed using affinity purification of protein corresponding to the bait eIF4E gene, using RT-PCR (real time polymerase chain reaction) to identify the copurifying RNA correspond to the hit gene NFKBIZ

Curated By

BioGRID

Interaction Summary

EIF4E

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA cap binding [TAS]poly(A) RNA binding [IDA]protein binding [IPI]translation initiation factor activity [IDA, ISS]

Gene Ontology Cellular Component

NFKBIZ