DST1
Gene Ontology Biological Process
- mRNA cleavage [IDA, IMP]
- maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter [IGI, IMP]
- positive regulation of RNA polymerase II transcriptional preinitiation complex assembly [IDA, IMP]
- positive regulation of transcription elongation from RNA polymerase II promoter [IDA]
- regulation of mRNA 3'-end processing [IGI, IMP]
- tRNA transcription from RNA polymerase III promoter [IMP]
- transcription antitermination [IDA]
- transcription elongation from RNA polymerase I promoter [IDA]
- transcription elongation from RNA polymerase II promoter [IDA, IMP]
- transcription from RNA polymerase III promoter [IDA]
- transcription initiation from RNA polymerase II promoter [IDA, IGI, IMP]
Gene Ontology Molecular Function
RPB9
Gene Ontology Biological Process
- cellular response to DNA damage stimulus [IMP]
- maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter [IGI, IMP]
- transcription from RNA polymerase II promoter [IMP]
- transcription initiation from RNA polymerase II promoter [IMP]
- transcription-coupled nucleotide-excision repair [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PCA
A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.
Publication
An in vivo map of the yeast protein interactome.
Protein interactions regulate the systems-level behavior of cells; thus, deciphering the structure and dynamics of protein interaction networks in their cellular context is a central goal in biology. We have performed a genome-wide in vivo screen for protein-protein interactions in Saccharomyces cerevisiae by means of a protein-fragment complementation assay (PCA). We identified 2770 interactions among 1124 endogenously expressed proteins. Comparison ... [more]
Throughput
- High Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| RPB9 DST1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| RPB9 DST1 | Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene. | Low | - | BioGRID | 162100 | |
| DST1 RPB9 | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | High | - | BioGRID | 661561 | |
| DST1 RPB9 | Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene. | Low | - | BioGRID | 238599 | |
| RPB9 DST1 | Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell. | Low | - | BioGRID | 439046 | |
| RPB9 DST1 | Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition. | Low | - | BioGRID | 425775 | |
| DST1 RPB9 | Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene. | Low | - | BioGRID | 439047 |
Curated By
- BioGRID