BAIT
KLF4
EZF, GKLF, RP11-150J11.1
Kruppel-like factor 4 (gut)
GO Process (26)
GO Function (9)
GO Component (2)
Gene Ontology Biological Process
- cellular response to growth factor stimulus [IDA]
- cellular response to laminar fluid shear stress [IMP]
- fat cell differentiation [ISS]
- mesodermal cell fate determination [TAS]
- negative regulation of NF-kappaB transcription factor activity [IDA]
- negative regulation of cell migration involved in sprouting angiogenesis [IDA]
- negative regulation of cell proliferation [TAS]
- negative regulation of chemokine (C-X-C motif) ligand 2 production [IDA]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA]
- negative regulation of heterotypic cell-cell adhesion [IDA]
- negative regulation of inflammatory response [TAS]
- negative regulation of interleukin-8 biosynthetic process [IDA]
- negative regulation of response to cytokine stimulus [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- positive regulation of cellular protein metabolic process [IMP]
- positive regulation of hemoglobin biosynthetic process [IMP]
- positive regulation of nitric oxide biosynthetic process [IMP]
- positive regulation of protein metabolic process [IGI]
- positive regulation of telomerase activity [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IGI, IMP, ISS]
- positive regulation of transcription, DNA-templated [ISS, NAS]
- post-embryonic hemopoiesis [IMP]
- regulation of cell differentiation [ISS]
- stem cell maintenance [ISS]
- transcription from RNA polymerase II promoter [ISS]
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- RNA polymerase II transcription factor binding [IPI, ISS]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA]
- core promoter proximal region sequence-specific DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [NAS]
- sequence-specific DNA binding transcription factor recruiting transcription factor activity [ISS]
- transcription regulatory region DNA binding [ISS]
- zinc ion binding [NAS]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IMP]
- RNA polymerase II transcription factor binding [IPI, ISS]
- RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription [IDA]
- core promoter proximal region sequence-specific DNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [NAS]
- sequence-specific DNA binding transcription factor recruiting transcription factor activity [ISS]
- transcription regulatory region DNA binding [ISS]
- zinc ion binding [NAS]
Gene Ontology Cellular Component
Homo sapiens
PREY
TRAF6
MGC:3310, RNF85
TNF receptor-associated factor 6, E3 ubiquitin protein ligase
GO Process (46)
GO Function (11)
GO Component (12)
Gene Ontology Biological Process
- Fc-epsilon receptor signaling pathway [TAS]
- I-kappaB kinase/NF-kappaB signaling [TAS]
- JNK cascade [TAS]
- MyD88-dependent toll-like receptor signaling pathway [TAS]
- MyD88-independent toll-like receptor signaling pathway [TAS]
- T cell receptor signaling pathway [IMP, TAS]
- TRIF-dependent toll-like receptor signaling pathway [TAS]
- activation of MAPK activity [TAS]
- activation of NF-kappaB-inducing kinase activity [IMP]
- activation of protein kinase activity [IDA]
- apoptotic signaling pathway [TAS]
- cellular response to lipopolysaccharide [IDA]
- innate immune response [TAS]
- membrane protein intracellular domain proteolysis [TAS]
- negative regulation of apoptotic process [TAS]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [IMP]
- neurotrophin TRK receptor signaling pathway [TAS]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- nucleotide-binding oligomerization domain containing signaling pathway [TAS]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [IDA, TAS]
- positive regulation of JUN kinase activity [IDA, NAS]
- positive regulation of NF-kappaB transcription factor activity [IDA, IMP, TAS]
- positive regulation of T cell activation [IC]
- positive regulation of T cell cytokine production [IMP]
- positive regulation of apoptotic process [TAS]
- positive regulation of interleukin-2 production [IMP]
- positive regulation of osteoclast differentiation [IDA]
- positive regulation of protein ubiquitination [NAS]
- positive regulation of sequence-specific DNA binding transcription factor activity [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IDA, NAS]
- positive regulation of transcription regulatory region DNA binding [IDA]
- protein K63-linked ubiquitination [IDA, IGI]
- protein autoubiquitination [IDA, TAS]
- protein polyubiquitination [IDA]
- response to interleukin-1 [IDA]
- stress-activated MAPK cascade [TAS]
- toll-like receptor 10 signaling pathway [TAS]
- toll-like receptor 2 signaling pathway [TAS]
- toll-like receptor 3 signaling pathway [TAS]
- toll-like receptor 4 signaling pathway [TAS]
- toll-like receptor 5 signaling pathway [TAS]
- toll-like receptor 9 signaling pathway [TAS]
- toll-like receptor TLR1:TLR2 signaling pathway [TAS]
- toll-like receptor TLR6:TLR2 signaling pathway [TAS]
- toll-like receptor signaling pathway [TAS]
Gene Ontology Molecular Function- histone deacetylase binding [IPI]
- mitogen-activated protein kinase kinase kinase binding [IPI]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- protein kinase B binding [IPI]
- protein kinase binding [IPI]
- thioesterase binding [IPI]
- tumor necrosis factor receptor binding [IPI]
- ubiquitin conjugating enzyme binding [IDA]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [EXP, IDA, TAS]
- histone deacetylase binding [IPI]
- mitogen-activated protein kinase kinase kinase binding [IPI]
- protein N-terminus binding [IPI]
- protein binding [IPI]
- protein kinase B binding [IPI]
- protein kinase binding [IPI]
- thioesterase binding [IPI]
- tumor necrosis factor receptor binding [IPI]
- ubiquitin conjugating enzyme binding [IDA]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [EXP, IDA, TAS]
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Polo-Like Kinase 1 phosphorylates and stabilizes KLF4 to promote tumorigenesis in nasopharyngeal carcinoma.
Rationale: Advanced nasopharyngeal carcinoma (NPC) is an aggressive disease with no targeted therapies and poor outcomes. New innovative targets are urgently needed. KLF4 has been extensively studied in the context of tumors, and current data suggest that it can act as either a tissue-specific tumor-inhibiting or a tumor-promoting gene. Here, we found that KLF4 played as a tumor-promoting gene in ... [more]
Theranostics Jul. 10, 2019; 9(12);3541-3554 [Pubmed: 31281496]
Throughput
- Low Throughput
Curated By
- BioGRID