BAIT
PLEKHA4
PEPP1
pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 4
GO Process (0)
GO Function (1)
GO Component (0)
Gene Ontology Molecular Function
Homo sapiens
PREY
GPX1
GPXD, GSHPX1
glutathione peroxidase 1
GO Process (21)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
- UV protection [IMP]
- arachidonic acid metabolic process [TAS]
- cell redox homeostasis [IDA]
- cellular response to oxidative stress [NAS]
- glutathione metabolic process [IDA]
- heart contraction [IMP]
- hydrogen peroxide catabolic process [IDA]
- lipoxygenase pathway [TAS]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IMP]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [IMP]
- negative regulation of release of cytochrome c from mitochondria [IMP]
- nucleobase-containing small molecule metabolic process [TAS]
- positive regulation of fibril organization [ISS]
- purine nucleobase metabolic process [TAS]
- purine nucleotide catabolic process [TAS]
- regulation of gene expression, epigenetic [IDA]
- regulation of mammary gland epithelial cell proliferation [IMP]
- regulation of proteasomal protein catabolic process [IDA]
- response to hydrogen peroxide [IMP]
- response to selenium ion [IMP]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
PLEKHA4/kramer Attenuates Dishevelled Ubiquitination to Modulate Wnt and Planar Cell Polarity Signaling.
Wnt signaling pathways direct key physiological decisions in development. Here, we establish a role for a pleckstrin homology domain-containing protein, PLEKHA4, as a modulator of signaling strength in Wnt-receiving cells. PLEKHA4 oligomerizes into clusters at PI(4,5)P2-rich regions of the plasma membrane and recruits the Cullin-3 (CUL3) E3 ubiquitin ligase substrate adaptor Kelch-like protein 12 (KLHL12) to these assemblies. This recruitment decreases ... [more]
Cell Rep Dec. 14, 2018; 27(7);2157-2170.e8 [Pubmed: 31091453]
Throughput
- High Throughput
Additional Notes
- assayed using SILAC IP (stable isotope labeling by amino acids in cell culture-based quantitative immunoprecipitation)
- only hits identified in both pull-downs have been included
Curated By
- BioGRID