BAIT

TTF2

HuF2, ZGRF6
transcription termination factor, RNA polymerase II
GO Process (3)
GO Function (2)
GO Component (1)
Homo sapiens
PREY

CLPB

HSP78, SKD3
ClpB caseinolytic peptidase B homolog (E. coli)
GO Process (1)
GO Function (0)
GO Component (0)

Gene Ontology Biological Process

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

hLodestar/HuF2 interacts with CDC5L and is involved in pre-mRNA splicing.

Leonard D, Ajuh P, Lamond AI, Legerski RJ

hLodestar/HuF2 belongs to the SNF2 family of proteins. This family of proteins has been shown to play a critical role in altering protein-DNA interactions in a variety of cellular contexts. We have identified an unexpected interaction between hLodestar/HuF2 and CDC5L in both the yeast two-hybrid system and HeLa nuclear extract. CDC5L is a well-characterized pre-mRNA splicing factor in yeast and ... [more]

Biochem. Biophys. Res. Commun. Sep. 05, 2003; 308(4);793-801 [Pubmed: 12927788]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CLPB TTF2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.8821BioGRID
1184353

Curated By

  • BioGRID