Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Characterization of the interaction of the monomeric GTP-binding protein Rab3a with geranylgeranyl transferase II.

Johannes L, Perez F, Laran-Chich MP, Henry JP, Darchen F

The monomeric GTP-binding protein Rab3a controls exocytosis in neuroendocrine and neuronal cells. Like other members of the Rab family, Rab3a is posttranslationally modified by the addition of hydrophobic geranylgeranyl groups to its C-terminus. The geranylgeranylation reaction is catalysed by the heterotrimeric geranylgeranyl transferase II. We describe the cDNA cloning of the beta-subunit of human geranylgeranyl transferase II by means of ... [more]

Eur. J. Biochem. Jul. 15, 1996; 239(2);362-8 [Pubmed: 8706741]

Throughput

  • Low Throughput

Curated By

  • BioGRID