BAIT

ORF7B

SARS-CoV2 ORF7b, SARS-CoV-2 ORF7b, 7b, NS7B_SARS2, PRO_0000449799, GU280_gp08
Protein non-structural 7b
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

BSCL2

GNG3LG, HMN5, PELD, SPG17
Berardinelli-Seip congenital lipodystrophy 2 (seipin)
GO Process (4)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Multilevel proteomics reveals host perturbations by SARS-CoV-2 and SARS-CoV.

Stukalov A, Girault V, Grass V, Karayel O, Bergant V, Urban C, Haas DA, Huang Y, Oubraham L, Wang A, Hamad MS, Piras A, Hansen FM, Tanzer MC, Paron I, Zinzula L, Enghleitner T, Reinecke M, Lavacca TM, Ehmann R, Woelfel R, Jores J, Kuster B, Protzer U, Rad R, Ziebuhr J, Thiel V, Scaturro P, Mann M, Pichlmair A

The global emergence of SARS-CoV-2 urgently requires an in-depth understanding of molecular functions of viral proteins and their interactions with the host proteome. Several individual omics studies have extended our knowledge of COVID-19 pathophysiology1-10. Integration of such datasets to obtain a holistic view of virus-host interactions and to define the pathogenic properties of SARS-CoV-2 is limited by the heterogeneity of ... [more]

Nature Apr. 12, 2021; (); [Pubmed: 33845483]

Quantitative Score

  • 9.099764107 [Confidence Score]

Throughput

  • High Throughput

Additional Notes

  • Affinity capture-MS was carried out using HA-tagged viral proteins as baits in human A549 lung carcinoma cells. Significant interactions were identified as those where the prey protein was at least 4 times enriched against the background (median log2 score > 2) with a p-value <= 1E-3.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ORF7B BSCL2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
2893585
ORF7B BSCL2
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

High-BioGRID
3753570
ORF7B BSCL2
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

High-BioGRID
3768874

Curated By

  • BioGRID