BAIT

ORF7B

SARS-CoV2 ORF7b, SARS-CoV-2 ORF7b, 7b, NS7B_SARS2, PRO_0000449799, GU280_gp08
Protein non-structural 7b
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

BSCL2

GNG3LG, HMN5, PELD, SPG17
Berardinelli-Seip congenital lipodystrophy 2 (seipin)
GO Process (4)
GO Function (0)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Comprehensive analysis of the host-virus interactome of SARS-CoV-2

Chen Z, Wang C, Feng X, Nie L, Tang M, Zhang H, Xiong Y, Swisher SK, Srivastava M, Chen J

Host-virus protein-protein interaction is the key component of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lifecycle. We conducted a comprehensive interactome study between the virus and host cells using tandem affinity purification and proximity labeling strategies and identified 437 human proteins as the high-confidence interacting proteins. Functional characterization and further validation of these interactions elucidated how distinct SARS-CoV-2 viral ... [more]

Status: Preliminary Report

Throughput

  • High Throughput

Additional Notes

  • SFB-TAP: Tandem affinity purification (TAP) with the SFB (S-protein, FLAG epitope, and streptavidin-binding peptide) tag
  • The identified prey proteins were filtered using Significance Analysis of INTeractome (SAINTexpress) and those with a Bayesian false discovery rate of 0.05 or less were considered high-confidence interacting proteins (HCIPs).

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ORF7B BSCL2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High9.0998BioGRID
2756112
ORF7B BSCL2
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

High-BioGRID
3753570
ORF7B BSCL2
PCA
PCA

A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay.

High-BioGRID
3768874

Curated By

  • BioGRID