SIGMAR1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
OPRM1
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger [TAS]
- behavioral response to ethanol [IMP]
- cellular response to morphine [IBA]
- cellular response to stress [IMP]
- negative regulation of Wnt protein secretion [IMP]
- negative regulation of adenylate cyclase activity [ISS]
- negative regulation of cAMP-mediated signaling [IDA]
- negative regulation of cell proliferation [TAS]
- negative regulation of cytosolic calcium ion concentration [IDA]
- negative regulation of nitric oxide biosynthetic process [IDA]
- neuropeptide signaling pathway [IMP]
- phospholipase C-activating G-protein coupled receptor signaling pathway [ISS]
- positive regulation of ERK1 and ERK2 cascade [ISS]
- positive regulation of cAMP-mediated signaling [IDA]
- positive regulation of cytosolic calcium ion concentration [IDA]
- positive regulation of neurogenesis [ISS]
- positive regulation of nitric oxide biosynthetic process [IDA]
- regulation of N-methyl-D-aspartate selective glutamate receptor activity [ISS]
- sensory perception [NAS]
- sensory perception of pain [IBA, ISS]
- synaptic transmission [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Sigma 1 receptor modulation of G-protein-coupled receptor signaling: potentiation of opioid transduction independent from receptor binding.
sigma Ligands modulate opioid actions in vivo, with agonists diminishing morphine analgesia and antagonists enhancing the response. Using human BE(2)-C neuroblastoma cells that natively express opioid receptors and human embryonic kidney (HEK) cells transfected with a cloned mu opioid receptor, we now demonstrate a similar modulation of opioid function, as assessed by guanosine 5'-O-(3-[(35)S]thio)triphosphate ([(35)S]GTP gamma S) binding, by sigma(1) ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| OPRM1 SIGMAR1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID