BAIT

MGAT1

GLCNAC-TI, GLCT1, GLYT1, GNT-1, GNT-I, MGAT
mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
Homo sapiens
PREY

C1GALT1

C1GALT, T-synthase
core 1 synthase, glycoprotein-N-acetylgalactosamine 3-beta-galactosyltransferase 1
Homo sapiens

Phenotypic Enhancement

A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

A CRISPR Screen Using Subtilase Cytotoxin Identifies SLC39A9 as a Glycan-Regulating Factor.

Yamaji T, Hanamatsu H, Sekizuka T, Kuroda M, Iwasaki N, Ohnishi M, Furukawa JI, Yahiro K, Hanada K

Subtilase cytotoxin (SubAB) is a virulence factor produced by locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli strains. The toxin recognizes sialoglycans for entry and cleaves an endoplasmic reticulum chaperon, binding immunoglobulin protein, to cause cell death. However, no systematic screening has yet been performed to identify critical host factors. Here, we performed a genome-wide CRISPR/Cas9 knockout screen for SubAB-induced cell ... [more]

iScience May. 31, 2019; 15();407-420 [Pubmed: 31108395]

Throughput

  • Low Throughput

Ontology Terms

  • biological process: response to toxic substance (GO:0009636)

Additional Notes

  • CRISPR method
  • double mutants show increased resistance to Subtilase cytotoxin (SubAB)

Curated By

  • BioGRID