PPP1CB
Gene Ontology Biological Process
- G2/M transition of mitotic cell cycle [TAS]
- circadian regulation of gene expression [ISS]
- entrainment of circadian clock by photoperiod [ISS]
- mitotic cell cycle [TAS]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- protein dephosphorylation [ISS]
- regulation of cell adhesion [IDA]
- regulation of circadian rhythm [IMP]
- small molecule metabolic process [TAS]
- transforming growth factor beta receptor signaling pathway [TAS]
- triglyceride catabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HOXA2
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
HOXA2 activity regulation by cytoplasmic relocation, protein stabilization and post-translational modification.
HOX proteins are homeodomain transcription factors critically involved in patterning animal embryos and controlling organogenesis. While the functions of HOX proteins and the processes under their control begin to be well documented, the modalities of HOX protein activity regulation remain poorly understood. Here we show that HOXA2 interacts with PPP1CB, a catalytic subunit of the Ser/Thr PP1 phosphatase complex. This ... [more]
Throughput
- Low Throughput
Additional Notes
- assayed using GST (glutathione S-transferase) pull-down experiments from lysates of human cell expressing the indicated proteins
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PPP1CB HOXA2 | PCA PCA A Protein-Fragment Complementation Assay (PCA) is a protein-protein interaction assay in which a bait protein is expressed as fusion to one of the either N- or C- terminal peptide fragments of a reporter protein and prey protein is expressed as fusion to the complementary N- or C- terminal fragment of the same reporter protein. Interaction of bait and prey proteins bring together complementary fragments, which can then fold into an active reporter, e.g. the split-ubiquitin assay. | Low | - | BioGRID | 2757756 |
Curated By
- BioGRID