Publication

Long noncoding RNA GAS5 inhibits progression of colorectal cancer by interacting with and triggering YAP phosphorylation and degradation and is negatively regulated by the m6A reader YTHDF3.

Ni W, Yao S, Zhou Y, Liu Y, Huang P, Zhou A, Liu J, Che L, Li J

YAP activation is crucial for cancer development including colorectal cancer (CRC). Nevertheless, it remains unclear whether N6-Methyladenosine (m6A) modified transcripts of long noncoding RNAs (lncRNAs) can regulate YAP activation in cancer progression. We investigated the functional link between lncRNAs and the m6A modification in YAP signaling and CRC progression.YAP interacting lncRNAs were screened by RIP-sequencing, RNA FISH and immunofluorescence co-staining ... [more]

Throughput

• Low Throughput

Additional Notes

• the interaction between GAS5 RNA and YAP protein was detected in two ways: (1) biotinylated RNA corresponding to bait gene GAS5 was mixed with cell lysates and pulled down and protein corresponding to the hit gene YAP was identified as an associated protein by western blotting and possibly by mass spectrometry; (2) assayed using BLI (biolayer interferometry) with GAS5 RNA immobilized on the chip and subsequent YAP protein association detected

Related interactions

Curated By

• BioGRID