SCN5A
Gene Ontology Biological Process
- AV node cell to bundle of His cell communication [IMP]
- SA node cell to atrial cardiac muscle cell communication [IMP]
- brainstem development [ISS]
- bundle of His cell to Purkinje myocyte communication [IMP]
- cardiac muscle cell action potential involved in contraction [IMP]
- cardiac muscle contraction [IMP]
- cardiac ventricle development [ISS]
- cellular response to calcium ion [IDA]
- cerebellum development [ISS]
- membrane depolarization [IDA]
- membrane depolarization during SA node cell action potential [ISS]
- membrane depolarization during action potential [IDA]
- membrane depolarization during cardiac muscle cell action potential [IMP]
- neuronal action potential [IBA]
- odontogenesis of dentin-containing tooth [ISS]
- positive regulation of action potential [ISS]
- positive regulation of epithelial cell proliferation [ISS]
- positive regulation of sodium ion transport [IDA]
- regulation of atrial cardiac muscle cell membrane depolarization [IMP]
- regulation of atrial cardiac muscle cell membrane repolarization [IMP]
- regulation of cardiac muscle cell contraction [IMP]
- regulation of heart rate [IMP]
- regulation of heart rate by cardiac conduction [IMP]
- regulation of sodium ion transmembrane transport [IDA]
- regulation of ventricular cardiac muscle cell membrane depolarization [IMP]
- regulation of ventricular cardiac muscle cell membrane repolarization [IMP]
- response to denervation involved in regulation of muscle adaptation [ISS]
- sodium ion transmembrane transport [IDA, IMP]
- sodium ion transport [IDA]
- telencephalon development [ISS]
- ventricular cardiac muscle cell action potential [IMP]
Gene Ontology Molecular Function- ankyrin binding [IDA]
- calmodulin binding [IPI]
- enzyme binding [IPI]
- fibroblast growth factor binding [IPI]
- ion channel binding [IPI]
- nitric-oxide synthase binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- scaffold protein binding [IPI]
- ubiquitin protein ligase binding [IPI]
- voltage-gated sodium channel activity [IDA]
- voltage-gated sodium channel activity involved in cardiac muscle cell action potential [IDA, IMP]
- ankyrin binding [IDA]
- calmodulin binding [IPI]
- enzyme binding [IPI]
- fibroblast growth factor binding [IPI]
- ion channel binding [IPI]
- nitric-oxide synthase binding [IPI]
- protein binding [IPI]
- protein kinase binding [IPI]
- scaffold protein binding [IPI]
- ubiquitin protein ligase binding [IPI]
- voltage-gated sodium channel activity [IDA]
- voltage-gated sodium channel activity involved in cardiac muscle cell action potential [IDA, IMP]
Gene Ontology Cellular Component
NEDD4
Gene Ontology Biological Process
- cellular response to UV [IMP]
- cytokine-mediated signaling pathway [TAS]
- development involved in symbiotic interaction [IMP]
- glucocorticoid receptor signaling pathway [IDA]
- lysosomal transport [IDA]
- negative regulation of sodium ion transport [IDA]
- negative regulation of transcription from RNA polymerase II promoter in response to UV-induced DNA damage [IMP]
- negative regulation of vascular endothelial growth factor receptor signaling pathway [ISS]
- neuron projection development [IEP]
- positive regulation of nucleocytoplasmic transport [IDA]
- positive regulation of phosphatidylinositol 3-kinase signaling [IMP]
- positive regulation of protein catabolic process [IDA]
- progesterone receptor signaling pathway [IDA]
- protein K63-linked ubiquitination [ISS]
- protein targeting to lysosome [IDA]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA, IMP]
- receptor catabolic process [IDA]
- receptor internalization [IDA]
- regulation of dendrite morphogenesis [ISS]
- regulation of ion transmembrane transport [IDA]
- regulation of membrane potential [IDA]
- regulation of potassium ion transmembrane transporter activity [IDA]
- response to calcium ion [TAS]
- transmission of virus [IMP]
- ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway [IMP]
Gene Ontology Molecular Function- RNA polymerase binding [IPI]
- beta-2 adrenergic receptor binding [IDA]
- phosphoserine binding [ISS]
- phosphothreonine binding [ISS]
- proline-rich region binding [IMP, IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sodium channel inhibitor activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin-protein transferase activity [IDA]
- RNA polymerase binding [IPI]
- beta-2 adrenergic receptor binding [IDA]
- phosphoserine binding [ISS]
- phosphothreonine binding [ISS]
- proline-rich region binding [IMP, IPI]
- protein binding [IPI]
- protein domain specific binding [IPI]
- sodium channel inhibitor activity [IDA]
- ubiquitin binding [IDA]
- ubiquitin-protein transferase activity [IDA]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Functional Consequences of the SCN5A-p.Y1977N Mutation within the PY Ubiquitylation Motif: Discrepancy between HEK293 Cells and Transgenic Mice.
Dysfunction of the cardiac sodium channel Nav1.5 (encoded by the SCN5A gene) is associated with arrhythmias and sudden cardiac death. SCN5A mutations associated with long QT syndrome type 3 (LQT3) lead to enhanced late sodium current and consequent action potential (AP) prolongation. Internalization and degradation of Nav1.5 is regulated by ubiquitylation, a post-translational mechanism that involves binding of the ubiquitin ... [more]
Throughput
- Low Throughput
Additional Notes
- assayed using GST (glutathione S-transferase) pull-down experiments
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NEDD4 SCN5A | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 478986 | |
| SCN5A NEDD4 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - | |
| SCN5A NEDD4 | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | - |
Curated By
- BioGRID