BAIT

KLF4

EZF, GKLF, RP11-150J11.1
Kruppel-like factor 4 (gut)
GO Process (26)
GO Function (9)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The deubiquitinase USP10 regulates KLF4 stability and suppresses lung tumorigenesis.

Wang X, Xia S, Li H, Wang X, Li C, Chao Y, Zhang L, Han C

Krueppel-like factor 4 (KLF4), a key transcription factor, acts as a multifunctional player involved in the progression of numerous aggressive cancers. The proteasome-dependent pathway is one of the main modalities in controlling KLF4 abundance at a posttranslational level. Although some of the ubiquitin ligases have been identified, the deubiquitinases of KLF4 and the regulatory function remain unexplored. Here, by screening ... [more]

Cell Death Differ. Jun. 01, 2020; 27(6);1747-1764 [Pubmed: 31748695]

Throughput

  • Low Throughput

Curated By

  • BioGRID