An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Functional analysis of human mitochondrial receptor Tom20 for protein import into mitochondria.

Yano M, Kanazawa M, Terada K, Takeya M, Hoogenraad N, Mori M

The mitochondrial import receptor translocase of the outer membrane of mitochondria (Tom20) consists of five segments, an N-terminal membrane-anchor segment, a linker segment rich in charged amino acids, a tetratricopeptide repeat motif, a glutamine-rich segment, and a C-terminal segment. To assess the role of each segment, four C-terminally truncated mutants of the human receptor (hTom20) were constructed, and the effect ... [more]

J. Biol. Chem. Oct. 09, 1998; 273(41);26844-51 [Pubmed: 9756929]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TOMM20

Gene Ontology Biological Process

Gene Ontology Molecular Function

P-P-bond-hydrolysis-driven protein transmembrane transporter activity [IDA]protein binding [IPI]unfolded protein binding [IDA]

Gene Ontology Cellular Component

OTC