BAIT

RPS6KC1

RPK118, RSKL1, S6K-delta-1, S6PKh1, humS6PKh1
ribosomal protein S6 kinase, 52kDa, polypeptide 1
GO Process (1)
GO Function (1)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens
PREY

SPHK1

SPHK
sphingosine kinase 1
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Identification and characterization of RPK118, a novel sphingosine kinase-1-binding protein.

Hayashi S, Okada T, Igarashi N, Fujita T, Jahangeer S, Nakamura S

Sphingosine kinase (SPHK) is a key enzyme catalyzing the formation of sphingosine 1 phosphate (SPP), a lipid messenger that is implicated in the regulation of a wide variety of important cellular events through intracellular as well as extracellular mechanisms. However, the molecular mechanism of the intracellular actions of SPP remains unclear. Here we have cloned a novel sphingosine kinase-1 (SPHK1)-binding ... [more]

J. Biol. Chem. Sep. 06, 2002; 277(36);33319-24 [Pubmed: 12077123]

Throughput

  • Low Throughput

Curated By

  • BioGRID