BAIT

MECR

CGI-63, FASN2B, NRBF1, RP11-467D18.1
mitochondrial trans-2-enoyl-CoA reductase
GO Process (1)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

PPARA

NR1C1, PPAR, PPARalpha, hPPAR, SC22CB-5E3.5
peroxisome proliferator-activated receptor alpha
GO Process (23)
GO Function (13)
GO Component (2)

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors.

Masuda N, Yasumo H, Furusawa T, Tsukamoto T, Sadano H, Osumi T

To identify the proteins which may modulate the functions of peroxisome proliferator-activated receptor (PPAR), a rat liver cDNA library was screened by a yeast two-hybrid system, using the mouse PPARalpha as a bait. A protein named nuclear receptor binding factor-1 (NRBF-1) was identified, which interacts not only with PPARalpha, but also with various nuclear hormone receptors in the presence of ... [more]

Gene Oct. 23, 1998; 221(2);225-33 [Pubmed: 9795230]

Throughput

  • Low Throughput

Curated By

  • BioGRID