BAIT

TRAF3

CAP-1, CAP1, CD40bp, CRAF1, IIAE5, LAP1
TNF receptor-associated factor 3
UPS Project
Homo sapiens
PREY

RNF144B

IBRDC2, PIR2, bA528A10.3, p53RFP
ring finger protein 144B
UPS Project
GO Process (1)
GO Function (2)
GO Component (3)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

RNF144B inhibits LPS-induced inflammatory responses via binding TBK1.

Zhang Z, Zhang L, Wang B, Zhu X, Zhao L, Chu C, Guo Q, Wei R, Yin X, Zhang Y, Li X

Innate immune responses need to be precisely controlled to avoid prolonged inflammation and prevent unwanted damage to the host. Here, we report that RNF144B responded dynamically to LPS stimulation and negatively regulated LPS-induced inflammation. We found that RNF144B interacted with the scaffold/dimerization domain (SDD) of TANK binding kinase 1 (TBK1) through the in between RING (IBR) domain to inhibit its ... [more]

J. Leukoc. Biol. Dec. 01, 2018; 106(6);1303-1311 [Pubmed: 31509299]

Throughput

  • Low Throughput

Additional Notes

  • Sources of TRAF3 and RNF144B not clear

Curated By

  • BioGRID