BAIT
CYP1A2
CP12, P3-450, P450(PA)
cytochrome P450, family 1, subfamily A, polypeptide 2
GO Process (18)
GO Function (8)
GO Component (2)
Gene Ontology Biological Process
- alkaloid metabolic process [IDA]
- arachidonic acid metabolic process [TAS]
- drug catabolic process [IMP]
- drug metabolic process [IDA]
- epoxygenase P450 pathway [TAS]
- exogenous drug catabolic process [IDA]
- heterocycle metabolic process [IDA]
- methylation [TAS]
- monocarboxylic acid metabolic process [IDA]
- monoterpenoid metabolic process [IDA]
- omega-hydroxylase P450 pathway [TAS]
- oxidation-reduction process [IDA]
- oxidative deethylation [IDA]
- oxidative demethylation [IDA]
- small molecule metabolic process [TAS]
- steroid catabolic process [IMP]
- toxin biosynthetic process [IDA]
- xenobiotic metabolic process [TAS]
Gene Ontology Molecular Function- caffeine oxidase activity [IDA]
- demethylase activity [IDA]
- electron carrier activity [TAS]
- enzyme binding [IPI]
- heme binding [IDA]
- monooxygenase activity [IDA]
- oxidoreductase activity [IDA]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen [IMP]
- caffeine oxidase activity [IDA]
- demethylase activity [IDA]
- electron carrier activity [TAS]
- enzyme binding [IPI]
- heme binding [IDA]
- monooxygenase activity [IDA]
- oxidoreductase activity [IDA]
- oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen [IMP]
Gene Ontology Cellular Component
Homo sapiens
PREY
CORO1B
CORONIN-2
coronin, actin binding protein, 1B
GO Process (10)
GO Function (4)
GO Component (9)
Gene Ontology Biological Process
- actin cytoskeleton organization [IBA]
- actin filament branching [IDA]
- actin filament bundle assembly [IDA]
- cell migration [IDA]
- endothelial cell chemotaxis [IDA]
- negative regulation of Arp2/3 complex-mediated actin nucleation [IDA]
- positive regulation of lamellipodium morphogenesis [IDA]
- protein localization to cell leading edge [IDA]
- ruffle organization [IDA]
- wound healing [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): TX100-soluble fractions from cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in parallel experiments using unfractionated cells or TX100-insoluble fractions
Curated By
- BioGRID