BAIT
PDIA6
ERP5, P5, TXNDC7
protein disulfide isomerase family A, member 6
GO Process (6)
GO Function (2)
GO Component (4)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PRKAG2
AAKG, AAKG2, CMH6, H91620p, WPWS
protein kinase, AMP-activated, gamma 2 non-catalytic subunit
GO Process (20)
GO Function (7)
GO Component (4)
Gene Ontology Biological Process
- ATP biosynthetic process [TAS]
- carnitine shuttle [TAS]
- cell cycle arrest [TAS]
- cellular lipid metabolic process [TAS]
- energy reserve metabolic process [TAS]
- glycogen metabolic process [IMP]
- insulin receptor signaling pathway [TAS]
- intracellular signal transduction [IMP]
- membrane organization [TAS]
- negative regulation of protein kinase activity [IDA]
- negative regulation of protein serine/threonine kinase activity [IDA]
- positive regulation of peptidyl-threonine phosphorylation [IMP]
- positive regulation of protein kinase activity [IMP]
- regulation of fatty acid biosynthetic process [TAS]
- regulation of fatty acid metabolic process [IMP]
- regulation of fatty acid oxidation [TAS]
- regulation of glucose import [TAS]
- regulation of glycolytic process [IMP]
- small molecule metabolic process [TAS]
- sterol biosynthetic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): TX100-soluble fractions from cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in parallel experiments using unfractionated cells or TX100-insoluble fractions
Curated By
- BioGRID