BAIT
VIM
CTRCT30, HEL113, RP11-124N14.1
vimentin
GO Process (4)
GO Function (7)
GO Component (9)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
PTGIS
CYP8, CYP8A1, PGIS, PTGI
prostaglandin I2 (prostacyclin) synthase
GO Process (16)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
- apoptotic signaling pathway [IDA]
- arachidonic acid metabolic process [TAS]
- cellular response to hypoxia [IDA]
- cellular response to interleukin-1 [IEP]
- cellular response to interleukin-6 [IEP]
- cyclooxygenase pathway [TAS]
- icosanoid metabolic process [TAS]
- negative regulation of NF-kappaB transcription factor activity [IDA]
- negative regulation of inflammatory response [IDA]
- negative regulation of nitric oxide biosynthetic process [IDA]
- positive regulation of angiogenesis [IMP]
- positive regulation of execution phase of apoptosis [IDA]
- positive regulation of peroxisome proliferator activated receptor signaling pathway [IDA]
- prostaglandin biosynthetic process [IDA]
- small molecule metabolic process [TAS]
- xenobiotic metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Histone Interaction Landscapes Visualized by Crosslinking Mass Spectrometry in Intact Cell Nuclei.
Cells organize their actions partly through tightly controlled protein-protein interactions-collectively termed the interactome. Here we use crosslinking mass spectrometry (XL-MS) to chart the protein-protein interactions in intact human nuclei. Overall, we identified ∼8,700 crosslinks, of which 2/3 represent links connecting distinct proteins. From these data, we gain insights on interactions involving histone proteins. We observed that core histones on the ... [more]
Mol. Cell Proteomics Dec. 01, 2017; 17(10);2018-2033 [Pubmed: 30021884]
Throughput
- High Throughput
Additional Notes
- interaction identified using XL-MS (cross-linking mass spectrometry): TX100-insoluble fractions from cells were treated with cross-linker and cross-linked proteins were identified by mass-spectrometry; interaction is undirectional; therefore bait and prey/hit have been assigned arbitrarily; interactions with FDRs (false discovery rates) of 1% or less were reported; this interaction was not detected in parallel experiments using unfractionated cells or TX100-soluble fractions
Curated By
- BioGRID