HSP90AA1
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- Fc-gamma receptor signaling pathway involved in phagocytosis [TAS]
- G2/M transition of mitotic cell cycle [TAS]
- axon guidance [TAS]
- chaperone-mediated protein complex assembly [IDA]
- innate immune response [TAS]
- mitochondrial transport [TAS]
- mitotic cell cycle [TAS]
- nitric oxide metabolic process [TAS]
- positive regulation of nitric oxide biosynthetic process [ISS]
- protein import into mitochondrial outer membrane [IDA]
- protein refolding [TAS]
- regulation of nitric-oxide synthase activity [TAS]
- response to unfolded protein [NAS]
- signal transduction [NAS]
- small molecule metabolic process [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
MYOD1
Gene Ontology Biological Process
- cellular response to estradiol stimulus [ISS]
- histone H3 acetylation [ISS]
- histone H4 acetylation [ISS]
- muscle cell differentiation [TAS]
- muscle cell fate commitment [ISS]
- muscle organ development [TAS]
- myotube cell development [IDA]
- positive regulation of muscle cell differentiation [IDA, TAS]
- positive regulation of myoblast fusion [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISS]
- protein phosphorylation [TAS]
- regulation of RNA splicing [IDA]
- regulation of transcription from RNA polymerase II promoter [TAS]
- skeletal muscle tissue development [TAS]
- transcription from RNA polymerase II promoter [TAS]
Gene Ontology Molecular Function- E-box binding [ISS]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- chromatin DNA binding [ISS]
- core promoter binding [ISS]
- nuclear hormone receptor binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [ISS]
- transcription coactivator activity [TAS]
- E-box binding [ISS]
- RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity [TAS]
- RNA polymerase II regulatory region sequence-specific DNA binding [IDA]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- chromatin DNA binding [ISS]
- core promoter binding [ISS]
- nuclear hormone receptor binding [IPI]
- protein binding [IPI]
- protein heterodimerization activity [ISS]
- transcription coactivator activity [TAS]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Conformational activation of a basic helix-loop-helix protein (MyoD1) by the C-terminal region of murine HSP90 (HSP84).
A murine cardiac lambda gt11 expression library was screened with an amphipathic helix antibody, and a recombinant representing the C-terminal 194 residues of murine HSP90 (HSP84) was cloned. Both recombinant and native HSP90s were then found to rapidly convert a basic helix-loop-helix protein (MyoD1) from an inactive to an active conformation, as assayed by sequence-specific DNA binding. The conversion process ... [more]
Throughput
- Low Throughput
Additional Notes
- crosslinking
Curated By
- BioGRID