BAIT

TIMP3

HSMRK222, K222, K222TA2, SFD, RP1-309I22.1
TIMP metallopeptidase inhibitor 3
GO Process (2)
GO Function (2)
GO Component (3)
Homo sapiens
PREY

ADAM17

ADAM18, CD156B, CSVP, NISBD, NISBD1, TACE
ADAM metallopeptidase domain 17
GO Process (41)
GO Function (7)
GO Component (11)

Gene Ontology Biological Process

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

The C-terminal domains of TACE weaken the inhibitory action of N-TIMP-3.

Lee MH, Verma V, Maskos K, Becherer JD, Knaeuper V, Dodds P, Amour A, Murphy G

Tumor necrosis factor-alpha converting enzyme (TACE) is an ADAM (a disintegrin and metalloproteinases) that comprises an active catalytic domain and several C-terminal domains. We compare the binding affinity and association rate constants of the N-terminal domain form of wild-type tissue inhibitor of metalloproteinase (TIMP-3; N-TIMP-3) and its mutants against full-length recombinant TACE and the truncated form of its catalytic domain. ... [more]

FEBS Lett. Jun. 05, 2002; 520(1);102-6 [Pubmed: 12044879]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
TIMP3 ADAM17
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High0.9967BioGRID
3178363

Curated By

  • BioGRID