ATRX
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- DNA damage response, signal transduction by p53 class mediator [ISS]
- DNA duplex unwinding [TAS]
- DNA methylation [TAS]
- DNA recombination [TAS]
- DNA replication-independent nucleosome assembly [IMP]
- cellular response to hydroxyurea [ISS]
- chromatin remodeling [IDA]
- negative regulation of telomeric RNA transcription from RNA pol II promoter [ISS]
- nucleosome assembly [IDA]
- positive regulation of nuclear cell cycle DNA replication [ISS]
- positive regulation of telomere maintenance [ISS]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of transcription, DNA-templated [TAS]
- replication fork processing [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SMC1A
Gene Ontology Biological Process
- DNA repair [TAS]
- RNA splicing [TAS]
- gene expression [TAS]
- mRNA splicing, via spliceosome [TAS]
- meiotic nuclear division [ISS]
- mitotic cell cycle [TAS]
- mitotic cell cycle checkpoint [IDA]
- mitotic sister chromatid cohesion [TAS]
- mitotic sister chromatid segregation [TAS]
- mitotic spindle organization [TAS]
- negative regulation of DNA endoreduplication [IMP]
- response to radiation [IEP]
- signal transduction in response to DNA damage [IDA]
- sister chromatid cohesion [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Synthetic Lethality
A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.
Publication
Genome-Wide CRISPR-Cas9 Screen Reveals Selective Vulnerability of ATRX-Mutant Cancers to WEE1 Inhibition.
The tumor suppressor gene ATRX is frequently mutated in a variety of tumors including gliomas and liver cancers, which are highly unresponsive to current therapies. Here, we performed a genome-wide synthetic lethal screen, using CRISPR-Cas9 genome editing, to identify potential therapeutic targets specific for ATRX-mutated cancers. In isogenic hepatocellular carcinoma (HCC) cell lines engineered for ATRX loss, we identified 58 ... [more]
Throughput
- Low Throughput
Ontology Terms
- phenotype: growth abnormality (HP:0001507) [viability (PATO:0000169)]
Additional Notes
- CRISPR GI screen
- Cell Line: PLC-PRF-5 (Hepatoma Cell Line)
- Experimental Setup: Timecourse
- GIST: A-phenotypic negative genetic interaction
- Library: GeckoV2
- Significance Threshold: Authors looked for genes which We then searched for genes targeted by the sgRNAs that were represented at nearly the same level at T0 as at T21 in ATRX WT cells, but depleted in ATRX KO cells at T21 compared with T0. Under these stringent criteria, we identified 58 genes, which were essential for viability in ATRX KO cells but nonessential for ATRX WT cells, as potential synthetic lethal partners for ATRX and signified genes that met this criteria in a hit list in the supp file
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ATRX SMC1A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID