BAIT

DHR2

YKL078W
Predominantly nucleolar DEAH-box ATP-dependent RNA helicase; required for 18S rRNA synthesis
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)
PREY

KRR1

L000004098, YCL059C
Nucleolar protein required for rRNA synthesis and ribosomal assembly; required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit; essential gene
Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The SSU processome interactome in Saccharomyces cerevisiae reveals novel protein subcomplexes.

Vincent NG, Charette JM, Baserga SJ

Ribosome assembly is an evolutionarily conserved and energy intensive process required for cellular growth, proliferation, and maintenance. In yeast, assembly of the small ribosomal subunit (SSU) requires approximately 75 assembly factors that act in coordination to form the SSU processome, a 6 MDa ribonucleoprotein complex. The SSU processome is required for processing, modifying, and folding the preribosomal RNA (rRNA) to ... [more]

RNA Dec. 01, 2017; 24(1);77-89 [Pubmed: 29054886]

Throughput

  • High Throughput

Additional Notes

  • Table S3

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KRR1 DHR2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
KRR1 DHR2
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

High-BioGRID
2789550

Curated By

  • BioGRID