BAIT

RRP3

RNA-dependent ATPase RRP3, L000004225, YHR065C
Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
GO Process (1)
GO Function (2)
GO Component (1)
Saccharomyces cerevisiae (S288c)
PREY

ESF1

YDR365C
Nucleolar protein involved in pre-rRNA processing; depletion causes severely decreased 18S rRNA levels
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The SSU processome interactome in Saccharomyces cerevisiae reveals novel protein subcomplexes.

Vincent NG, Charette JM, Baserga SJ

Ribosome assembly is an evolutionarily conserved and energy intensive process required for cellular growth, proliferation, and maintenance. In yeast, assembly of the small ribosomal subunit (SSU) requires approximately 75 assembly factors that act in coordination to form the SSU processome, a 6 MDa ribonucleoprotein complex. The SSU processome is required for processing, modifying, and folding the preribosomal RNA (rRNA) to ... [more]

RNA Dec. 01, 2017; 24(1);77-89 [Pubmed: 29054886]

Throughput

  • High Throughput

Additional Notes

  • Table S3

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ESF1 RRP3
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
-
ESF1 RRP3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.2205BioGRID
1927552

Curated By

  • BioGRID