BAIT

UTP5

YDR398W
Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit
Saccharomyces cerevisiae (S288c)
PREY

UTP21

YLR409C
Subunit of U3-containing 90S preribosome and SSU processome complexes; involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma; Small Subunit processome is also known as SSU processome
GO Process (2)
GO Function (0)
GO Component (5)
Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The SSU processome interactome in Saccharomyces cerevisiae reveals novel protein subcomplexes.

Vincent NG, Charette JM, Baserga SJ

Ribosome assembly is an evolutionarily conserved and energy intensive process required for cellular growth, proliferation, and maintenance. In yeast, assembly of the small ribosomal subunit (SSU) requires approximately 75 assembly factors that act in coordination to form the SSU processome, a 6 MDa ribonucleoprotein complex. The SSU processome is required for processing, modifying, and folding the preribosomal RNA (rRNA) to ... [more]

RNA Dec. 01, 2017; 24(1);77-89 [Pubmed: 29054886]

Throughput

  • High Throughput

Additional Notes

  • Table S3

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
UTP5 UTP21
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High9BioGRID
3595787
UTP21 UTP5
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.3167BioGRID
1945358

Curated By

  • BioGRID