YBX1
Gene Ontology Biological Process
Gene Ontology Molecular Function- DNA binding [IDA, TAS]
- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- double-stranded DNA binding [TAS]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
- DNA binding [IDA, TAS]
- RNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]
- double-stranded DNA binding [TAS]
- poly(A) RNA binding [IDA]
- protein binding [IPI]
- sequence-specific DNA binding transcription factor activity [TAS]
Gene Ontology Cellular Component
- CRD-mediated mRNA stability complex [IDA]
- U12-type spliceosomal complex [IDA]
- cytoplasm [IDA]
- cytoplasmic stress granule [IDA]
- extracellular vesicular exosome [IDA]
- histone pre-mRNA 3'end processing complex [ISS]
- intracellular membrane-bounded organelle [IDA]
- nuclear membrane [IDA]
- nucleoplasm [TAS]
- nucleus [TAS]
- ribonucleoprotein complex [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
RNA splicing mediated by YB-1 is inhibited by TLS/CHOP in human myxoid liposarcoma cells.
Human myxoid liposarcoma contains a characteristic t(12;16) chromosomal translocation that results in fusion of the N-terminal domain of the translocated in liposarcoma (TLS) protein to the C/EBP homologous protein (CHOP). TLS possesses structural motifs that suggest it may participate in RNA processing. We demonstrate that in human myxoid liposarcoma cells, wild-type TLS binds to RNA polymerase II (Pol II) via ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| FUS YBX1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| FUS YBX1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | - | BioGRID | 926778 |
Curated By
- BioGRID