BAIT

ORF14

SARS-CoV2 ORF14, SARS-CoV-2 ORF14, 14, Y14_SARS2, PRO_0000449658, GU280_gp14
ORF14 protein
COVID-19 Coronavirus Project
GO Process (0)
GO Function (0)
GO Component (0)
Severe acute respiratory syndrome coronavirus 2
PREY

SMU1

BWD, SMU-1, fSAP57, RP11-54K16.3
smu-1 suppressor of mec-8 and unc-52 homolog (C. elegans)
Cell Cycle - Mitotic Spindle Assembly Project
UPS Project
GO Process (0)
GO Function (1)
GO Component (0)

Gene Ontology Molecular Function

Homo sapiens

Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

A SARS-CoV-2 - host proximity interactome

Samavarchi-Tehrani P, Abdouni H, Knight JD, Astori A, Samson R, Lin Z, Kim D, Knapp JJ, St- Germain J, Go CD, Larsen B, Wong CJ, Cassonnet P, Demeret C, Jacob Y, Roth FP, Raught B, Gingras A

Viral replication is dependent on interactions between viral polypeptides and host proteins. Identifying virus-host protein interactions can thus uncover unique opportunities for interfering with the virus life cycle via novel drug compounds or drug repurposing. Importantly, many viral-host protein interactions take place at intracellular membranes and poorly soluble organelles, which are difficult to profile using classical biochemical purification approaches. Applying ... [more]

Status: Preliminary Report

Quantitative Score

  • 4.5 [CLSC]

Throughput

  • High Throughput

Additional Notes

  • BioID
  • SAINTexpress (v.3.6.1) was used to identify viral-host proximity interactions and those with an FDR =< 0.01 were considered high confidence. Control-subtracted and length-adjusted spectral counts (CLSC) were calculated and the top viral-host protein interactions are represented by a higher CLSC score.

Curated By

  • BioGRID