BAIT

HRAS

C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
Harvey rat sarcoma viral oncogene homolog
GO Process (39)
GO Function (3)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

PIK3CD

APDS, IMD14, P110DELTA, PI3K, p110D, RP11-558F24.3
phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit delta
GO Process (32)
GO Function (5)
GO Component (4)
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The junctional multidomain protein AF-6 is a binding partner of the Rap1A GTPase and associates with the actin cytoskeletal regulator profilin.

Boettner B, Govek EE, Cross J, Van Aelst L

The AF-6 protein is a multidomain protein that contains two potential Ras-binding domains within its N terminus. Because of this feature, AF-6 has been isolated in both two-hybrid and biochemical approaches and is postulated to be a potential Ras-effector protein. Herein, we show that it is specifically the first Ras-binding domain of AF-6 that mediates this interaction and that the ... [more]

Proc. Natl. Acad. Sci. U.S.A. Aug. 01, 2000; 97(16);9064-9 [Pubmed: 10922060]

Throughput

  • Low Throughput

Curated By

  • BioGRID