MP
Gene Ontology Biological Process
- embryo development ending in seed dormancy [IGI]
- flower development [IGI]
- leaf vascular tissue pattern formation [IGI]
- longitudinal axis specification [IMP]
- meristem development [IGI]
- regulation of transcription, DNA-templated [IEP]
- response to auxin [IEP]
- root development [IGI]
- xylem and phloem pattern formation [IMP]
Gene Ontology Molecular Function
NPH4
Gene Ontology Biological Process
- blue light signaling pathway [IMP]
- gravitropism [TAS]
- lateral root development [IGI]
- lateral root formation [IGI]
- leaf development [IGI]
- phototropism [IMP]
- positive regulation of transcription, DNA-templated [IMP]
- regulation of growth [TAS]
- regulation of transcription, DNA-templated [TAS]
- response to auxin [IMP]
- response to ethylene [IGI]
Gene Ontology Molecular Function
FRET
An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.
Publication
Fluorescence cross-correlation analyses of the molecular interaction between an Aux/IAA protein, MSG2/IAA19, and protein-protein interaction domains of auxin response factors of arabidopsis expressed in HeLa cells.
Since auxin may elicit numerous developmental responses by the use of a combination of auxin response factors (ARFs) and their Aux/IAA repressors, it is important to determine the interaction between the two protein families in a quantitative manner. We transiently expressed the C-terminal protein-protein interaction domains (CTDs) of Arabidopsis ARFs, MP/ARF5 and NPH4/ARF7, and MSG2/IAA19, fused to fluorescent proteins in ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
MP NPH4 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - | |
NPH4 MP | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID