BAIT

MP

ARF5, AUXIN RESPONSE FACTOR 5, F6F9.10, F6F9_10, IAA24, MONOPTEROS, TRANSCRIPTION FACTOR IAA24, indole-3-acetic acid inducible 24, AT1G19850
auxin response factor 5
Arabidopsis thaliana (Columbia)
PREY

NPH4

ARF7, AUXIN RESPONSE FACTOR 7, AUXIN-REGULATED TRANSCRIPTIONAL ACTIVATOR 7, AUXIN-RESPONSIVE TRANSCRIPTIONAL ACTIVATOR 7, BIP, BIPOSTO, IAA21, IAA23, IAA25, MASSUGU 1, MSG1, NON-PHOTOTROPHIC HYPOCOTYL, T1M15.130, T1M15_130, TIR5, TRANSPORT INHIBITOR RESPONSE 5, indole-3-acetic acid inducible 21, indole-3-acetic acid inducible 23, indole-3-acetic acid inducible 25, AT5G20730
auxin-regulated transcriptional activator NPH4
Arabidopsis thaliana (Columbia)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Overlapping and non-redundant functions of the Arabidopsis auxin response factors MONOPTEROS and NONPHOTOTROPIC HYPOCOTYL 4.

Hardtke CS, Ckurshumova W, Vidaurre DP, Singh SA, Stamatiou G, Tiwari SB, Hagen G, Guilfoyle TJ, Berleth T

Transcription factors of the auxin response factor (ARF) family have been implicated in auxin-dependent gene regulation, but little is known about the functions of individual ARFs in plants. Here, interaction assays, expression studies and combinations of multiple loss- and gain-of-function mutants were used to assess the roles of two ARFs, NONPHOTOTROPIC HYPOCOTYL 4 (NPH4/ARF7) and MONOPTEROS (MP/ARF5), in Arabidopsis development. ... [more]

Development Mar. 01, 2004; 131(5);1089-100 [Pubmed: 14973283]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MP NPH4
FRET
FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Low-BioGRID
-
NPH4 MP
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID