Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
The tripartite motif family identifies cell compartments.
A functional genomic approach, based on systematic data gathering, was used to characterize a family of proteins containing a tripartite motif (TRIM). A total of 37 TRIM genes/proteins were studied, 21 of which were novel. The results demonstrate that TRIM proteins share a common function: by means of homo-multimerization they identify specific cell compartments. ... [more]
EMBO J. May. 01, 2001; 20(9);2140-51 [Pubmed: 11331580]
Throughput
- Low Throughput
Curated By
- BioGRID