AES
Gene Ontology Biological Process
- multicellular organismal development [TAS]
- negative regulation of canonical Wnt signaling pathway [IDA]
- negative regulation of gene expression [IMP]
- negative regulation of protein binding [IDA]
- negative regulation of response to cytokine stimulus [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of transcription, DNA-templated [IDA]
- organ morphogenesis [TAS]
- positive regulation of anoikis [IMP]
- response to interleukin-1 [IDA]
Gene Ontology Molecular Function
GTF2E1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Inhibition of androgen receptor-mediated transcription by amino-terminal enhancer of split.
A yeast two-hybrid assay has identified an androgen-dependent interaction of androgen receptor (AR) with amino-terminal enhancer of split (AES), a member of the highly conserved Groucho/TLE family of corepressors. Full-length AR, as well as the N-terminal fragment of AR, showed direct interactions with AES in in vitro protein-protein interaction assays. AES specifically inhibited AR-mediated transcription in a well-defined cell-free transcription ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID