Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.


Cloning and sequencing of a novel human gene which encodes a putative hydroxylase.

Iriyama C, Matsuda S, Katsumata R, Hamaguchi M

Using a conventional two-hybrid technique with MAWD as bait protein, a novel full-length cDNA was isolated and sequenced from a human liver cDNA library. This cDNA consists of 2,575 base pairs and has a predicted open reading frame encoding 255 amino acids. Overall, it is similar to the catalytic enzyme PHZF, catalyzing the hydroxylation of phenazine-1-carboxylic acid to 2-hydroxyphenazine-1-carboxylic acid. ... [more]

J. Hum. Genet. May. 17, 2001; 46(5);289-92 [Pubmed: 11355021]


  • Low Throughput

Curated By

  • BioGRID