An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

SH3GLB, a new endophilin-related protein family featuring an SH3 domain.

Pierrat B, Simonen M, Cueto M, Mestan J, Ferrigno P, Heim J

A new cDNA encoding a protein of 362 amino acids designated SH3GLB1, for SH3 domain GRB2-like endophilin B1, was identified in a yeast two-hybrid screen devoted to the identification of new partners interacting with the apoptosis inducer Bax. SH3GLB1 shows strong similarities to the SH3 domain-containing proteins of the endophilin family and presumably represents the human homologue of the potential ... [more]

Genomics Jan. 15, 2001; 71(2);222-34 [Pubmed: 11161816]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SH3GLB1 SH3GLB2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Behrends C (2010)	High	-	BioGRID	446513
SH3GLB2 SH3GLB1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Behrends C (2010)	High	-	BioGRID	446517
SH3GLB1 SH3GLB2	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cho NH (2022)	High	-	BioGRID	3369904
SH3GLB2 SH3GLB1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Cho NH (2022)	High	-	BioGRID	3369905
SH3GLB2 SH3GLB1	Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.	Huttlin EL (2021)	High	1	BioGRID	3162891
SH3GLB2 SH3GLB1	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Kristensen AR (2012)	High	-	BioGRID	925588
SH3GLB1 SH3GLB2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Blandin G (2013)	High	-	BioGRID	-
SH3GLB1 SH3GLB2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Luck K (2020)	High	-	BioGRID	2704159
SH3GLB1 SH3GLB2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Pierrat B (2001)	Low	-	BioGRID	-
SH3GLB2 SH3GLB1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Pierrat B (2001)	Low	-	BioGRID	-
SH3GLB1 SH3GLB2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Rolland T (2014)	High	-	BioGRID	-
SH3GLB2 SH3GLB1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Yachie N (2016)	High	-	BioGRID	-
SH3GLB1 SH3GLB2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Yachie N (2016)	High	-	BioGRID	-
SH3GLB2 SH3GLB1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Yang JS (2018)	High	-	BioGRID	2473909
SH3GLB1 SH3GLB2	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Yang JS (2018)	High	-	BioGRID	2473911

Curated By

BioGRID

Interaction Summary

SH3GLB1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]protein binding [IPI]protein homodimerization activity [IPI]

Gene Ontology Cellular Component

SH3GLB2