An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

LDL receptor-related protein as a component of the midkine receptor.

Muramatsu H, Zou K, Sakaguchi N, Ikematsu S, Sakuma S, Muramatsu T

Midkine (MK) is a heparin-binding growth factor with migration-promoting and survival-promoting activities. To identify signaling receptor(s) of MK, membrane glycoproteins with MK-binding activity were isolated from day 13 mouse embryos by lectin- and MK-affinity chromatography. SDS-PAGE followed by protein sequence analysis revealed the presence of LDL receptor-related protein (LRP) and NCAM in the fraction. The dissociation constant of binding between ... [more]

Biochem. Biophys. Res. Commun. Apr. 21, 2000; 270(3);936-41 [Pubmed: 10772929]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

MDK

Gene Ontology Biological Process

Gene Ontology Molecular Function

growth factor activity [NAS]heparin binding [IDA]

Gene Ontology Cellular Component

NCAM1