BAIT
MDK
ARAP, MK, NEGF2
midkine (neurite growth-promoting factor 2)
GO Process (6)
GO Function (2)
GO Component (1)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
TUBA1A
B-ALPHA-1, LIS3, TUBA3
tubulin, alpha 1a
GO Process (8)
GO Function (2)
GO Component (6)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
LDL receptor-related protein as a component of the midkine receptor.
Midkine (MK) is a heparin-binding growth factor with migration-promoting and survival-promoting activities. To identify signaling receptor(s) of MK, membrane glycoproteins with MK-binding activity were isolated from day 13 mouse embryos by lectin- and MK-affinity chromatography. SDS-PAGE followed by protein sequence analysis revealed the presence of LDL receptor-related protein (LRP) and NCAM in the fraction. The dissociation constant of binding between ... [more]
Biochem. Biophys. Res. Commun. Apr. 21, 2000; 270(3);936-41 [Pubmed: 10772929]
Throughput
- Low Throughput
Curated By
- BioGRID